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Effects of Machilus thunbergii Sieb et Zucc on UV‐induced photoaging in hairless mice
Author(s) -
Uhm Yoon Kyung,
Jung Kyung Hee,
Bu HeeJung,
Jung Mi Young,
Lee MuHyoung,
Lee Sun,
Lee Sunjoo,
Kim Hye Kyung,
Yim SungVin
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3117
Subject(s) - hairless , photoaging , superoxide dismutase , malondialdehyde , oxidative stress , chemistry , epidermis (zoology) , pinus thunbergii , pharmacology , biochemistry , dermatology , biology , medicine , botany , anatomy
Chronic ultraviolet (UV) exposure induces photoaging and oxidative stress in the skin. We investigated whether Machilus thunbergii Sieb et Zucc ( M. thunbergii ) could reduce UV‐induced photoaging and oxidative stress in hairless mice. The dorsal skin of hairless mice was treated topically with M. thunbergii for 2 h prior to UV irradiation. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were then measured in skin and/or serum samples. Histological changes in the skin were assessed by hematoxylin‐eosin (HE) staining. In addition, proteomes from the skin of hairless mice in each group were analyzed. The thickness of the dorsal skin and epidermis was significantly decreased by M. thunbergii treatment. We also found that MDA levels decreased after M. thunbergii treatment and the SOD levels were increased by M. thunbergii compared with those in the UV‐only treated group. Proteomic analysis revealed 17 proteins associated with photoaging. These data indicate that M. thunbergii might have antiphotoaging effects. Copyright © 2010 John Wiley & Sons, Ltd.