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Pharmacokinetics of bilobalide, ginkgolide A and B after administration of three different Ginkgo biloba L. preparations in humans
Author(s) -
Woelkart Karin,
Feizlmayr Elisabeth,
Dittrich Peter,
Beubler Eckhard,
Pinl Fritz,
Suter Andy,
Bauer Rudolf
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3074
Subject(s) - ginkgo biloba , ginkgoales , pharmacokinetics , pharmacognosy , pharmacology , traditional medicine , ginkgo , medicine , chemistry , biological activity , biochemistry , in vitro
A sensitive LC‐ESI‐MS method with a solid‐phase extraction was established for the determination of bilobalide, ginkgolide A and ginkgolide B in human plasma; bioavailability and pharmacokinetics of three different Ginkgo biloba L. preparations have been investigated. The preparations used in the present single‐dose pharmacokinetic study were different formulations of Ginkgo biloba L. extracts (Geriaforce™ tincture, new Ginkgo fresh plant extract tablets and EGb 761™) with various excipients. The analysis of Ginkgo terpene lactones was performed by LC‐MS on a Zorbax ® SB‐C18 column. The mobile phase consisted of water + 0.1% acetic acid and methanol 68/32 (v/v) to 49/51 (v/v) at a flow rate of 200 μL/min. Bilobalide, ginkgolide A and ginkgolide B were monitored using the selected‐ion monitoring (SIM) mode at m/z of 325, 453 and 423, respectively. The amounts of the active compounds (terpene lactones) in the administered products were in the low‐mg range per dose. The assay method was successfully applied to the study of the pharmacokinetics and bioavailability of bilobalide, gingkolide A and ginkgolide B in humans. The resulting maximum concentrations (median) of bilobalide, ginkgolide A and ginkgolide B in plasma after administration of the maximum daily dose of the different Ginkgo products were 3.53, 3.62, and 1.38 ng/mL respectively after administration of Geriaforce™ tincture; 11.68, 7.36, and 4.18 ng/mL, respectively after taking Ginkgo fresh plant extract tablets; and 26.85, 16.44, 9.99 ng/mL, respectively after administration of EGb 761™ tablets. These data are relevant to demonstrate relative bioavailabilities of different Ginkgo biloba L. preparations (Geriaforce™ tincture, new Ginkgo fresh plant extract tablets and EGb 761™). Copyright © 2009 John Wiley & Sons, Ltd.