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Bioguided isolation of angiotensin‐converting enzyme inhibitors from the seeds of Plantago asiatica L.
Author(s) -
Geng Fang,
Yang Li,
Chou Guixin,
Wang Zhengtao
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3071
Subject(s) - chemistry , angiotensin converting enzyme , enzyme , fractionation , phenylpropanoid , glycoside , in vitro , traditional medicine , biochemistry , chromatography , stereochemistry , biosynthesis , biology , medicine , blood pressure , endocrinology
Ethanolic extract of the seeds of Plantago asiatica L. showed significant inhibitory activity of angiotensin‐converting enzyme (ACE) determined by monitoring the transformation from a substrate hippuryl‐histidyl‐leucine (HHL) to the product hippuric acid (HA) in vitro using an UPLC‐MS method. The bioguided fractionation of the extract resulted in the isolation of four ACE inhibitory active phenylpropanoid glycosides acteoside, isoacteoside, plantainoside D, and plantamajoside with IC 50 values of 2.69 mM, 2.46 mM, 2.17 mM, and 2.47 mM, respectively. Their structures were elucidated through the analysis of NMR, UV, IR and MS data. Our study is the first demonstration that Plantago asiatica L. and its major constituents have ACE inhibitory activity in vitro . It is assumed that the identified compounds contribute to the angiotensin‐converting enzyme‐inhibitory activity of the extract. Copyright © 2009 John Wiley & Sons, Ltd.