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Persicarin from water dropwort ( Oenanthe javanica ) protects primary cultured rat cortical cells from glutamate‐induced neurotoxicity
Author(s) -
Ma Choong Je,
Lee Ki Yong,
Jeong Eun Ju,
Kim Seung Hyun,
Park Junghyun,
Choi Yun Hee,
Kim Young Choong,
Sung Sang Hyun
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3065
Subject(s) - neuroprotection , glutathione , neurotoxicity , glutamate receptor , glutathione reductase , nitric oxide , biochemistry , antioxidant , glutathione peroxidase , pharmacology , chemistry , biology , toxicity , endocrinology , enzyme , receptor , organic chemistry
The n‐BuOH fraction of O. javanica significantly protected the primary cultures of rat cortical cells exposed to glutamate. Four flavonoids yielded from this fraction through bioactivity‐guidance. The isolated compounds, identified as isorhamnetin (1), afzelin (2), hyperoside (3) and persicarin (4), were evaluated in vitro for their neuroprotective activity. Persicarin (4), the main constituent of O. javanica , showed significant neuroprotective activities in glutamate‐injured rat cortical cells. Persicarin diminished calcium influx and inhibited the subsequent overproduction of nitric oxide and intracellular peroxide. In addition, persicarin significantly restored the reduced activities of glutathione (GSH) reductase and glutathione peroxidase, and the contents of GSH induced by glutamate. These results support a conclusion that persicarin greatly contributes to the neuroprotective activities of O. javanica . Copyright © 2009 John Wiley & Sons, Ltd.