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Antitumor activities of dammarane triterpene saponins from Bacopa monniera
Author(s) -
Peng Ling,
Zhou Yun,
Kong De Yun,
Zhang Wei Dong
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3034
Subject(s) - triterpene , cytotoxicity , chemistry , petroleum ether , in vivo , traditional medicine , mtt assay , saponin , in vitro , pharmacology , apocynaceae , stereochemistry , biochemistry , chromatography , biology , medicine , extraction (chemistry) , alternative medicine , microbiology and biotechnology , pathology
Bioassay‐guided methods were used to test the antitumor activity of methanol extract of the whole plant of Bacopa monniera (L.) Wettst. and four different fractions (petroleum ether, CHCl 3 , EtOAc, and n ‐BuOH fractions) of the methanol extract. Among the five crude samples, n ‐BuOH fraction was noted to have the highest antitumor activity. The dammarane triterpene saponins isolated from n ‐BuOH fraction, bacopaside É (1) and bacopaside VII (3), had potential antitumor effect. 1 and 3 showed cytotoxicity of all the tested human tumor cell lines MDA‐MB‐231, SHG‐44, HCT‐8, A‐549 and PC‐3M in MTT assay in vitro , and showed 90.52 % and 84.13 % inhibition in mouse implanted with sarcoma S180 in vivo at the concentration of 50 μmol/kg, respectively. The remaining two compounds, bacopaside II (2) and bacopasaponin C (4) were found to be much less potent compared with 1 and 3. 1 and 3 significantly inhibited human breast cancer cell line MDA‐MB‐231 adhesion, migration and Matrigel invasion in vitro at the concentration of 50 μmol/L. Since no antitumor activities about the monomers from Bacopa monniera (L.) Wettst. have been reported, these results indicate that the mechanism of action of 1 and 3 needs further study. Copyright © 2009 John Wiley & Sons, Ltd.