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Eutigoside C attenuates radiation‐induced crypt injury in the mouse intestine
Author(s) -
Moon Changjong,
Ahn Kyuyoung,
Kim Jihoon,
Kim Jeongtae,
Kim SungHo,
Oh Tae Heon,
Lee Nam Ho,
Jee Youngheun,
Hyun Jin Won,
Park Jae Woo,
Shin Taekyun
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.3029
Subject(s) - crypt , oxidative stress , superoxide dismutase , apoptosis , catalase , small intestine , pharmacology , antioxidant , chemistry , andrology , biology , medicine , biochemistry
On Jeju Island, South Korea, the leaves of Eurya emarginata have been traditionally used to treat ulcers or as a diuretic. Eutigoside C isolated from the leaves has been reported to have in vitro anti‐inflammatory effects. We evaluated the radioprotective effects of eutigoside C on jejunal cell apoptosis and crypt survival in mice subjected to gamma irradiation. In addition, the ability of eutigoside C to protect against radiation‐induced oxidative stress was examined by evaluating the activities of superoxide dismutase (SOD) and catalase (CAT) in radiation‐induced hepatic injury. Eutigoside C was administered intraperitoneally at 48, 12, and 1 h before irradiation. The administration of eutigoside C (10, 50, or 100 mg/kg body weight) before irradiation protected the intestinal crypts from radiation‐induced apoptosis ( p < 0.05), and attenuated radiation‐induced decrease of villous height ( p < 0.05). Pretreating mice prior to irradiation with eutigoside C (100 mg/kg) significantly improved the survival of the jejunal crypt ( p < 0.01). The dose reduction factor was 1.09 at 3.5 days after irradiation. Treatment of eutigoside C prior to irradiation significantly protected SOD and CAT activities in radiation‐induced hepatic injury ( p < 0.05). These results suggest that eutigoside C is a useful radioprotector capable of defending intestinal progenitor cells against indirect depletion, such as oxidative stress and inflammatory response caused by gamma irradiation. Copyright © 2009 John Wiley & Sons, Ltd.