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Ethanol extract of Fructus Ligustri Lucidi promotes osteogenesis of mesenchymal stem cells
Author(s) -
Li Guo,
Zhang Xiaoai,
Zhang Jinfang,
Chan Chuyan,
Yew David Tai Wai,
He Mingliang,
Lin Marie Chiami,
Leung Pingchung,
Kung Hsiangfu
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2987
Subject(s) - mesenchymal stem cell , alkaline phosphatase , osteoblast , osteoporosis , downregulation and upregulation , mineralization (soil science) , chemistry , microbiology and biotechnology , endocrinology , medicine , biology , biochemistry , enzyme , in vitro , gene , organic chemistry , nitrogen
Fructus Ligustri Lucidi (FLL) has been used in traditional Chinese medicine for over 1000 years. The ethanol extract of FLL (EFLL) has been shown to be a potential candidate in the prevention and treatment of osteoporosis. The present study aimed to determine whether EFLL carries out the effect by promoting osteogenesis in mesenchymal stem cells (MSCs). The osteogenic differentiation of MSCs was evaluated by their alkaline phosphatase (ALP) activities and mineralization. Expression of genes was detected by RT‐PCR. We found that EFLL significantly stimulated the ALP activities and shortened the time needed for the mineralization of MSCs during osteogenic differentiation. The expression of several osteoblast differentiation regulators was also upregulated by EFLL during this process. Our study demonstrated that the EFLL is capable of enhancing osteogenic differentiation of MSCs. It might be useful for treating diseases with inadequate bone formation, including osteoporosis. Copyright © 2009 John Wiley & Sons, Ltd.