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Protective effects of 3′‐deoxy‐4‐O‐methylepisappanol from Caesalpinia sappan against glutamate‐induced neurotoxicity in primary cultured rat cortical cells
Author(s) -
Moon HyungIn,
Chung IllMin,
Seo SuHyun,
Kang EunYoung
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2982
Subject(s) - neuroprotection , neurotoxicity , glutamate receptor , toxicity , pharmacology , chemistry , viability assay , biochemistry , biology , in vitro , organic chemistry , receptor
To examine the neuroprotective effects of Caesalpinia sappan L., we tested its protection against the glutamate‐induced neurotoxicity in primary cortical cultured neurons. We found that an aqueous extract of this medicinal plant exhibited significant protection against glutamate‐induced toxicity in primary cultured rat cortical cells. In order to clarify the neuroprotective mechanism(s) of this observed effect, isolation was performed to seek and identify active fractions and components. By such fractionation, two known compounds – sappanchalcone and 3′‐deoxy‐4‐O‐methylepisappanol – were isolated from the methanol extracts from the air‐dried and chipped C. sappan . Among these two compounds, 3′‐deoxy‐4‐O‐methylepisappanol exhibited significant neuroprotective activities against glutamate‐induced toxicity, exhibiting cell viability of about 50%, at concentrations ranging from 0.1 μM to 10 μM. Therefore, the neuroprotective effect of C. sappan might be due to the inhibition of glutamate‐induced toxicity by the protosappanin derivative it contains. Copyright © 2009 John Wiley & Sons, Ltd.