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Protective effect of bilberry ( Vaccinium myrtillus L.) extracts on cultured human corneal limbal epithelial cells (HCLEC)
Author(s) -
Song Juxian,
Li Yiqing,
Ge Jian,
Duan Yongheng,
Sze Stephen ChoWing,
Tong Yao,
Shaw PangChui,
Ng TziBun,
Tsui Kam Chuen,
Zhuo Yehong,
Zhang Kalin Yanbo
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2974
Subject(s) - bilberry , vaccinium myrtillus , viability assay , glycosaminoglycan , incubation , anthocyanin , vaccinium , hyaluronic acid , cytotoxicity , chemistry , cell , biology , in vitro , biochemistry , botany , food science , anatomy
The use of bilberry ( Vaccinium myrtillus L.) as a food and medicine for improving human vision has a long history all over the world. However, there is lack of convincing evidence from rigorous clinical trials or scientific research. This study investigated the effects of different concentrations of bilberry extracts on the cell viability, cell cycle and the expression of hyaluronic acid and glycosaminoglycans of cultured human corneal limbal epithelial cells. The data showed that bilberry extracts had no cytotoxicity to the corneal limbal epithelial cells at a wide range of concentrations (10 −9 –10 −4 M, equalized to the content of cyanidin‐3‐O‐glucoside). Bilberry extract (10 −6 , 10 −5 and 10 −4 M) increased cell viability after 48 h incubation. The number of cells decreased in G 0 /G 1 phase and increased prominently in S and G 2 /M phases after treatment with bilberry extracts at a high concentration (10 −4 M). The expression of glycosaminoglycans increased prominently after incubation with bilberry extracts (10 −7 and 10 −4 M) for 48 h while no significant changes were observed for the expression of hyaluronic acid. The results indicated that bilberry extract may be beneficial for the physiological renewal and homeostasis of corneal epithelial cells. Copyright © 2010 John Wiley & Sons, Ltd.