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Antiproliferative effect of benzophenones and their influence on cathepsin activity
Author(s) -
Murata Ramiro Mendonça,
Yatsuda Regiane,
dos Santos Marcelo Henrique,
Kohn Luciana K.,
Martins Felipe Terra,
Nagem Tanus Jorge,
Alencar Severino Matias,
de Carvalho João Ernesto,
Rosalen Pedro Luiz
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2954
Subject(s) - traditional medicine , chemistry , pharmacognosy , pharmacology , medicine , biological activity , biochemistry , in vitro
The antiproliferative activity of two prenylated benzophenones isolated from Rheedia brasiliensis , the triprenylated garciniaphenone and the tetraprenylated benzophenone 7‐epiclusianone, was investigated against human cancer cell lines. The antiproliferative activity on melanoma (UACC‐62), breast (MCF‐7), drug‐resistant breast (NCI‐ADR), lung/non‐small cells (NCI460), ovarian (OVCAR 03), prostate (PC03), kidney (786‐0), lung (NCI‐460) and tongue (CRL‐1624 and CRL‐1623) cancer cells was determined using spectrophotometric quantification of the cellular protein content. The effect of these benzophenones on the activity of cathepsins B and G was also investigated. Garciniaphenone displayed cytostatic activity in all cell lines, whereas 7‐epiclusianone showed a dose‐dependent cytotoxic effect. The IC 50 values for cell proliferation revealed that 7‐epiclusianone is more active than garciniaphenone against most of the cell lines. Furthermore, the antiproliferative effects demonstrated by garciniaphenone and 7‐epiclusianone were related to their cathepsin inhibiting properties. In conclusion, 7‐epiclusianone is a promising naturally occurring agent which displays multiple inhibitory effects which may be working in concert to inhibit cancer cell proliferation in vitro . The putative pathway by which 7‐epiclusianone affects cancer cell development may involve cathepsin inhibition. Copyright © 2009 John Wiley & Sons, Ltd.