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Antiviral and anticancer activities of 13( E )‐labd‐13‐ene‐8α,15‐diol isolated from Brachyglottis monroi
Author(s) -
Choi Hwa Jung,
Song Jae Hyoung,
Kwon Dur Han,
Baek Seung Hwa
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2875
Subject(s) - hela , cytotoxicity , cytopathic effect , ribavirin , stereochemistry , chemistry , diol , biochemistry , in vitro , organic chemistry , gene , genotype
The antiviral activity of 13( E )‐labd‐13‐ene‐8α,15‐diol (1), isolated from Brachyglottis monroi, was examined against human rhinovirus 2 (HRV2) and 3 (HRV3), and the anticancer activity on human cancer cells (A549 and Hep2). Compound (1) showed strong anti‐HRV2 and HRV3 activity with a 50% inhibitory concentration (IC 50 ) of 2.68 and 0.87 µg/mL, respectively, and a 50% cytotoxicity concentration (CC 50 ) of 59.45 µg/mL. Ribavirin only showed anti‐HRV3 activity with an IC 50 of 30.48 µg/mL and a CC 50 > 100 µg/mL. The addition of compound (1) to HRV‐infected HeLa cells directly reduced the formation of visible cytopathic effect (CPE) and it directly interacted with HRV particles. Furthermore, A549 and Hep2 cells incubated with 32 µg/mL of compound (1) for 48 h exhibited antilung and antilaryngeal cancer activities, with a viability of less than 50%. These results suggest that compound (1) may be used as a potential antiviral and anticancer agent. Copyright © 2009 John Wiley & Sons, Ltd.