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Downregulation of pro‐inflammatory cytokines by lupeol measured using cytometric bead array immunoassay
Author(s) -
Ahmad Sheikh Fayaz,
Pandey Anjali,
Kour Kiranjeet,
Bani Sarang
Publication year - 2010
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2844
Subject(s) - lupeol , proinflammatory cytokine , immunoassay , pharmacology , chemistry , immunology , medicine , chromatography , inflammation , biochemistry , antibody
The objective of the study was to investigate the activity of Lupeol (LUP) on proinflammatory and anti‐inflammatory cytokines in the pleural exudate from male swiss albino mice. We applied Cytometric bead array technology for simultaneously measurement of these cytokines in pleurisy induced mice treated with lupeol in graded oral doses. Cytometric bead array uses the sensitivity of amplified fluorescence detection by flowcytometer to measure soluble analytes in a particle based immune assay. This assay can accurately quantitate 5 cytokines in a 50 microlitre sample volume. Oral administration of LUP at doses of 25, 50, 100 and 200 mg/kg p.o. produced dose related inhibition of IL‐2, IFN‐gamma and TNF‐ α in the pleural exudate with the most significant effect at 100 mg/kg oral dose. LUP had a non significant inhibitory effect on the levels of IL‐4 and IL‐5. Copyright © 2009 John Wiley & Sons, Ltd.