Premium
Cytoprotective effect of lignans from Forsythia suspensa against peroxynitrite‐induced LLC‐PK 1 cell damage
Author(s) -
Piao Xiang Lan,
Cho Eun Jung,
Jang Moon Hee,
Cui Jian
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2834
Subject(s) - peroxynitrite , lignan , pharmacology , chemistry , antioxidant , traditional medicine , biochemistry , medicine , superoxide , stereochemistry , enzyme
There is mounting evidence that peroxynitrite (ONOO − ) is closely related to the pathogenesis of various diseases. As a pharmacological strategy aimed at preventing ONOO − ‐mediated toxicity, the protective activity of Forsythia suspensa (Thunb.) Vahl (Oleaceae) against ONOO − ‐induced cellular damage was investigated and its active components identified. After bioactivity‐guided fractionation of its methylene chloride fraction, two tetrahydrofurofuran lignans were isolated, namely phillygenin and 8‐hydroxypinoresinol. The protective effects of these lignans against ONOO − ‐induced cell death were evaluated using renal epithelial cell LLC‐PK1. Phillygenin and 8‐hydroxypinoresinol significantly reduced the cell injury by 3‐morpholinosydnonimine (SIN‐1), a ONOO − generator. The hydroxy substituents on the phenyl moieties may contribute to the antioxidant activities of these lignans. These results suggest that phillygenin and 8‐hydroxypinoresinol may be useful for the therapeutic or preventive applications in treating ONOO − ‐related diseases. Copyright © 2009 John Wiley & Sons, Ltd.