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Cytoprotective effect of Proanthocyanidin‐rich cranberry fraction against bacterial cell wall‐mediated toxicity in macrophages and epithelial cells
Author(s) -
La Vu Dang,
Labrecque Julie,
Grenier Daniel
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2799
Subject(s) - viability assay , proanthocyanidin , toxicity , cytotoxicity , lipopolysaccharide , cranberry juice , microbiology and biotechnology , monocyte , cell , chemistry , cell growth , polyphenol , biology , pharmacology , biochemistry , immunology , in vitro , antioxidant , organic chemistry , urinary system , endocrinology
Recent studies brought evidence regarding the potential beneficial effects of cranberry polyphenols for periodontal infections. In this study, we evaluated the capacity of a proanthocyanidin‐rich cranberry fraction to protect macrophages and oral epithelial cells against cytotoxicity induced by bacterial components. U937 cells, differentiated into adherent macrophage‐like cells, as well as oral epithelial cells were treated with cell wall or lipopolysaccharide preparations from periodontopathogens. Cell viability was monitored using a commercial MTT (3‐[4,5‐diethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide) assay. The cytoprotective effect was evaluated by pre‐incubating human cells with a proanthocyanidin‐rich cranberry fraction prior to treatment with the bacterial components at toxic concentrations. Among the various bacterial components tested, Peptostreptotoccus micros cell wall was found to be the most toxic for macrophages and epithelial cells and was thus selected for further analyses. Treatment of monocyte‐derived macrophages with cell wall of P. micros (20 µg/ml) decreased the cell viability by approximately 50%. Adding the cranberry fraction prior to treating cells with P. micros cell wall dose‐dependently protected monocyte‐derived macrophages from the toxic effect. A dose‐dependent cytoprotective effect of the cranberry fraction was also observed with oral epithelial cells treated with P. micros cell wall. This study suggests that cranberry polyphenols may exert a protective effect for host cells against the toxicity induced by bacterial components. Copyright © 2009 John Wiley & Sons, Ltd.