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Manassantin A and B from Saururus chinensis inhibiting cellular melanin production
Author(s) -
Seo ChangSeob,
Lee WonHee,
Chung HeeWook,
Chang Eun Ju,
Lee Seung Ho,
Jahng Yurngdong,
Hwang Bang Yeon,
Son JongKeun,
Han SangBae,
Kim Youngsoo
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2791
Subject(s) - melanin , ibmx , tyrosinase , hyperpigmentation , arbutin , skin whitening , chemistry , kojic acid , forskolin , biochemistry , biology , enzyme , pharmacology , in vitro , active ingredient
Abstract Hyperpigmentation disorders such as freckles and senile lentigines in the skin are associated with abnormal accumulation of melanin pigments. In this study, two lignan constituents were isolated from Saururus chinensis Baill (Saururaceae) as inhibitors of cellular melanin production by bioassay‐guided fractionations. The active constituents were manassantin A and B that dose‐dependently inhibited melanin production in α ‐melanocyte stimulating hormone ( α ‐MSH)‐activated melanoma B16 cells with IC 50 values of 13 n m and 8 n m , respectively. Arbutin as a positive control exhibited an IC 50 value of 96 µ m on α ‐MSH‐induced melanin production. Further, manassantin A inhibited forskolin‐ or 3‐isobutyl‐1‐methylxanthine (IBMX)‐induced melanin production with IC 50 values of 14 n m or 12 n m , respectively. Manassantin A decreased cellular amounts of IBMX‐inducible tyrosinase protein but could not affect the catalytic activity of cell‐free tyrosinase, a key enzyme in the biosynthetic pathway of melanin pigments. Finally, this study could provide a pharmacological potential of S. chinensis in hyperpigmentation disorders. Copyright © 2009 John Wiley & Sons, Ltd.