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Mechanism of the antiproliferative action of limonene on a lymphoma cell line: participation of nitric oxide. antiproliferative action of limonene on a lymphoma cell line
Author(s) -
Manuele Maria Gabriela,
Arcos Maria Laura Barreiro,
Davicino Roberto,
Ferraro Graciela,
Cremaschi Graciela,
Anesini Claudia
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2743
Subject(s) - nitric oxide , limonene , apoptosis , cell cycle , mechanism of action , cell culture , cell cycle checkpoint , lymphoma , chemistry , pharmacology , cancer research , biology , biochemistry , in vitro , immunology , genetics , endocrinology , food science , essential oil
Previously limonene demonstrated antiproliferative action on a lymphoma cell line (BW5147). The objective of this study was to investigate the mechanism of the antiproliferative action of limonene on BW 5147 cells. The induction of apoptosis and arrest in cell cycle phases, both phenomena related to nitric oxide (NO) production, was analyzed, as well as NO production. Results showed that limonene exhibited antiproliferative action on tumoral lymphocytes exerting a decrease in cell viability that was related to apoptosis induction and to the increase in NO levels at long incubation times. At short times and depending on its concentration, limonene arrested cells in different phases of the cell cycle, related to NO production. Copyright © 2009 John Wiley & Sons, Ltd.