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Inhibitory effects of green tea catechin on the lipid accumulation in 3T3‐L1 adipocytes
Author(s) -
Lee MakSoon,
Kim ChongTai,
Kim InHwan,
Kim Yangha
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2737
Subject(s) - catechin , green tea , chemistry , 3t3 l1 , adipocyte , pharmacognosy , biochemistry , inhibitory postsynaptic potential , biological activity , lipid accumulation , polyphenol , pharmacology , traditional medicine , food science , biology , antioxidant , in vitro , medicine , adipose tissue , endocrinology
The aim of the present study was to evaluate the effects of green tea (‐)‐epigallocatechin‐3‐gallate (EGCG) on the depletion of accumulated fat in differentiated 3T3‐L1 adipocytes. Intracellular lipid accumulation was decreased significantly after 24 h of incubation with 10 µ m EGCG, while the viability of adipocytes was reported to be unaffected. Under the same experimental conditions, the amount of glycerol released from cells into the medium was increased by 10 µ m EGCG. The level of mRNA in the 3T3‐L1 adipocytes was analysed by quantitative real‐time RT‐PCR. EGCG notably increased the mRNA level of hormone sensitive lipase (HSL), which catalyses the rate‐limiting stage in hydrolysis of stored triacylglycerol to monoacylglycerol and free fatty acids. In conclusion, the experiment produced results which showed that green tea EGCG effectively depleted fat accumulation via the stimulation of lipolysis and increased HSL gene expression in 3T3‐L1 adipocytes. These results may relate to the mechanism by which EGCG modulates lipolysis in adipocytes. Copyright © 2008 John Wiley & Sons, Ltd.