Premium
Effects of Microdesmis keayana alkaloids on vascular parameters of erectile dysfunction
Author(s) -
Zamblé Alexis,
MartinNizard Françoise,
Sahpaz Sevser,
Reynaert MarieLine,
Staels Bart,
Bordet Régis,
Duriez Patrick,
Gressier Bernard,
Bailleul François
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2717
Subject(s) - enos , superoxide , erectile dysfunction , phenylephrine , reactive oxygen species , nitric oxide , pharmacognosy , antioxidant , vasodilation , endothelial dysfunction , pharmacology , chemistry , dpph , biochemistry , traditional medicine , medicine , nitric oxide synthase , biological activity , in vitro , enzyme , blood pressure
Microdesmis keayana (Pandaceae) is an African tropical plant whose roots are used in traditional medicine for erection impairment but the compounds responsible for its action are unknown. Two major alkaloids recently isolated from the roots of M. keayana , keayanidine B and keayanine, were tested for vasorelaxing properties using isolated rat aortic rings precontracted by phenylephrine to confirm its traditional use. Influence of the alkaloids on the endothelial production of endothelial nitric oxide synthase (eNOS) was measured by quantitative polymerase chain reaction (QPCR) analysis. Scavenging activities were assessed versus 1,1‐diphenyl‐2‐picrylhydrazyle (DPPH) and reactive oxygen species (ROS) such as superoxide anion (O 2 •− ) and hydrogen peroxide (H 2 O 2 ) in cell‐free and cellular systems. The results showed that keayanidine B and keayanine had significant vasorelaxing properties. This effect could be due to their strong antioxidant activity versus O 2 •− and H 2 O 2 and to their stimulation of eNOS mRNA expression. Therefore these alkaloids could indirectly stimulate NO production in the vascular bed and would explain the traditional use of M. keayana in erectile dysfunction. Copyright © 2008 John Wiley & Sons, Ltd.