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Protective effect of daidzin against d ‐galactosamine and lipopolysaccharide‐induced hepatic failure in mice
Author(s) -
Kim SungHwa,
Heo JeongHaing,
Kim Yeong Shik,
Kang Sam Sik,
Choi Jae Sue,
Lee SunMee
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2710
Subject(s) - daidzin , lipopolysaccharide , puerarin , galactosamine , pharmacology , oxidative stress , chemistry , lipid peroxidation , biochemistry , medicine , immunology , endocrinology , daidzein , pathology , alternative medicine , genistein , glucosamine
This study examined the effects of daidzin, a major isoflavone from Puerariae Radix, on d ‐galactosamine ( d ‐GalN) and lipopolysaccharide (LPS)‐induced liver failure. Mice were given an intraperitoneal injection of daidzin (25, 50, 100 and 200 mg/kg) 1 h before receiving an injection of d ‐GalN (700 mg/kg)/LPS (10 µg/kg). Daidzin markedly reduced the elevated serum aminotransferase activity and the levels of lipid peroxidation and tumor necrosis factor‐ α . The glutathione content was lower in the d ‐GalN/LPS group, which was attenuated by daidzin. The daidzin pretreatment attenuated the swollen mitochondria observed in the d ‐GalN/LPS group. Daidzin attenuated the apoptosis of hepatocytes, which was confirmed using the terminal deoxynucleotidyl transferase‐mediated dUTP nick end‐labeling method and a caspase‐3 assay. Overall, these results suggest that the liver protection of daidzin is due to reduced oxidative stress and its antiapoptotic activity. Copyright © 2008 John Wiley & Sons, Ltd.