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Grape seed extract Vitis vinifera protects against radiation‐induced oxidative damage and metabolic disorders in rats
Author(s) -
Saada Helen N.,
Said Ussama Z.,
Meky Nefissa H.,
Azime Afrag S. Abd El
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2684
Subject(s) - oxidative stress , tbars , grape seed extract , ionizing radiation , reactive oxygen species , glutathione , chemistry , oxidative phosphorylation , pharmacology , antioxidant , medicine , endocrinology , biochemistry , irradiation , biology , lipid peroxidation , pathology , enzyme , alternative medicine , physics , nuclear physics
Whole body exposure to ionizing radiation induces the formation of reactive oxygen species (ROS) in different tissues provoking oxidative damage, organ dysfunction and metabolic disturbances. The present study was designed to determine the possible protective effect of grape seed extract (GSE), rich in proanthocyanidins against γ ‐radiation‐induced oxidative stress in heart and pancreas tissues associated with serum metabolic disturbances. Irradiated rats were whole body exposed to 5 Gy γ ‐radiation. GSE‐treated irradiated rats received 100 mg GSE/kg/day, by gavage, for 14 days before irradiation. The animals were killed on days 1, 14 and 28 after irradiation. Significant decreases of SOD, CAT and GSH‐Px activities associated with significant increases of TBARS levels were recorded in both tissues after irradiation. GSE administration pre‐irradiation significantly attenuated the radiation‐induced oxidative stress in heart tissues which was substantiated by a significant amelioration of serum LDH, CPK and AST activities. GSE treatment also attenuated the oxidative stress in pancreas tissues which was associated with a significant improvement in radiation‐induced hyperglycemia and hyperinsulinemia. In conclusion, the present data demonstrate that GSE would protect the heart and pancreas tissues from oxidative damage induced by ionizing irradiation. Copyright © 2008 John Wiley & Sons, Ltd.