Premium
Isolation and identification of hemostatic ingredients from lamiophlomis rotata (Benth.) kudo
Author(s) -
Li Maoxing,
Zhang Ruxue,
Jia Zhengping,
Sheng Jie,
Qiu Jianguo,
Wang Juan
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2669
Subject(s) - hemostasis , chemistry , high performance liquid chromatography , traditional medicine , bleeding time , glycoside , pharmacology , iridoid , active ingredient , hypericum perforatum , phytotherapy , chromatography , medicine , stereochemistry , platelet , platelet aggregation , surgery , immunology , alternative medicine , pathology
Abstract Lamiophlomis rotata (Benth.) Kudo (LR) is a traditional drug used by Chinese minorities such as Tibetans, Mongolians and Na Xi with the beneficial effects of hemostasis and alleviating pain. Flavonoids ingredients (P 1 ), iridoid glycosides ingredients (P 2 ) and maximus polarity components ingredients (P 3 ) were isolated from the aqueous extract of LR (AELR) with polyamide and macroporous adsorptive resins chromatographic columns. The hemostatic activity of these ingredients was estimated by the changes in bleeding time (BT), clotting time (CT) and blood coagulation parameters in mice or rats. RP‐HPLC was used to analyze the chemical composition of P 2 . As a result, AELR and P 2 significantly shortened BT, CT and thrombin time (TT), and increased fibrinogen (FIB), but P 1 and P 3 showed no hemostatic effect during the experiment. Moreover, P 2 showed satisfactory hemostatic effect with dose‐effect relationship and low toxicity in mice. 8‐Dehydroxy shanzhiside, phloyoside II, shanzhiside methylester, loganin, and 8‐ O ‐acetylshanzhiside methylester were detected as the main iridoid glycosides in P 2 by RP‐HPLC. Our results suggest that iridoid glycosides may be the hemostatic activity ingredients of L. rotata and are a potential hemostatic medicine. Copyright © 2009 John Wiley & Sons, Ltd.