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The immunomodulatory effect of saikosaponin derivatives and the root extract of Bupleurum kaoi in mice
Author(s) -
Yen MingHong,
Lin ChunChing,
Yen ChuanMin
Publication year - 1995
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650090509
Subject(s) - saponin , potency , stimulation , immune system , body weight , spleen , bupleurum , pharmacology , in vitro , chemistry , medicine , immunology , traditional medicine , endocrinology , biochemistry , traditional chinese medicine , pathology , alternative medicine
The saikosaponins ‐a(SSa), ‐d(SSd), ‐f(SSf), crude saponin mixture (CSM) and H 2 O‐layer components (HLC) which were isolated from the root of Bupleurum kaoi Liu, Chao et Chuang (Umbelliferae), demonstrated an immunomodulatory effect. Mice of the BALB/c strain were injected peritoneally with drugs to assess immune responses weekly over a 4 week period. The results showed that the mean ratio of thymus weight to body weight increased during week 1 but declined gradually thereafter; the ratio of helper T‐cells to suppressor T‐cells (Th/Ts) from mice treated with SSf and HLC gradually increased; however, in mice treated with SSa, SSd and CSM, the ratio rose to a maximum at week 3 and then decreased; the IL‐2 level of treated mice was significantly higher than that of the control group throughout the test period; the ratio of spleen weight to body weight in all drug treated groups increased weekly; the activity of B‐cell stimulation in mice was promoted week by week; levels of IgA, IgG and IgM in serum of SSd and SSa treated animals increased. An in vitro study, involving stimulation of IL‐1 demonstrated the following order of potency: SSd