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In vitro inhibition of adenosine deaminase by a group of steroid and Triterpenoid compounds
Author(s) -
Koch Heinrich P.,
Aichinger Andrea,
Bohne Bernd,
Plank Gerlinde
Publication year - 1994
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650080213
Subject(s) - sapogenin , adenosine deaminase , steroid , chemistry , enzyme , stereochemistry , adenosine , inhibitory postsynaptic potential , in vitro , biochemistry , biology , medicine , endocrinology , alternative medicine , pathology , hormone
The effects of several sapogenins and saponins of the steroid and triterpenoid classes, as well as of a few common phytosterols, have been tested for their inhibitory action towards adenosine deaminase (ADA). It was found that the acidic sapogenins strongly inhibit the enzyme. The mean inhibitory concentration (IC 50 ) was in the range 10 −3 to 10 −2 mg/mL, while the inhibitor constants ( K i ) have been estimated as about 10 −6 M. In contrast to this finding is the complete absence of any influence on the enzyme of the neutral sapo(ge)nins and the phytosterols. It is suggested that ADA is a worthwhile therapeutic target and that the (acidic) sapogenins, which are regular constituents of many medicinal plant species, presumably contribute their activity through an interaction with this enzyme.