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Effects of flavonoids isolated from licorice roots ( Glycyrrhiza inflata Bat.) On degranulation in human polymorphonuclear neutrophils
Author(s) -
Kimura Yoshiyuki,
Okuda Takuo,
Okuda Hiromichi
Publication year - 1993
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650070503
Subject(s) - degranulation , liquiritigenin , isoliquiritigenin , chemistry , glycyrrhiza , calcium , cytochalasin b , ionophore , pharmacology , platelet activating factor , biochemistry , immunology , biology , medicine , in vitro , alternative medicine , receptor , organic chemistry , pathology
The effects of chalcones and flavanone isolated from licorice roots ( Glycyrrhiza inflata Bat.) on human polymorphonuclear neutrophil (PMN) degranulation were investigated. Licochalcone A (2‐methoxy‐4,4′‐dihydroxy‐5‐α,α‐dimethylallylchalcone) and licochalcone B (2‐methoxy 3,4,4′‐trihydroxychalcone), at concentrations of 10 −6 M to 10 −3 M, inhibited calcium ionophore A 23187 (A 23187)‐, leukotriene B 4 (LTB 4 ) plus cytochalasin B (cyto B)‐, platelet activating factor (PAF) plus cyto B‐ and N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) plus cyto B‐induced lysosomal enzyme release from human PMNs. Isoliquiritigenin, isoliquiritin, liquiritigenin and liquiritin had no effects on PMN degranulation induced by various stimuli. In addition, licochalcones A and B, at high concentrations (10 −4 M–10 −3 M), increased cyclic AMP in human PMNs. Furthermore, licochalcones A and B, at concentrations of 10 −6 M to 10 −3 M, reduced the elevation of cytosolic free calcium concentration induced by calcium ionophore ionomycine (ionomycine), fMLP, PAF and LTB 4 .