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Modulation of microsomal activity by potential chemopreventive agents of plant origin
Author(s) -
Teel R. W.
Publication year - 1992
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650060506
Subject(s) - chemistry , microsome , catechin , carcinogen , biochemistry , ellagic acid , pyrene , metabolite , tannic acid , enzyme , antioxidant , polyphenol , organic chemistry
Ten compounds with potential chemopreventive activity were studied to determine their effects on microsomal mixed‐function oxidase and epoxide hydratase activity. In these studies, the highest nontoxic concentration of each compound was incubated with 1 mg rat liver microsomal protein and either [H 3 ]benzo[a]pyrene 4,5‐oxide or [H 3 ]benzo[a]pyrene 7,8‐dihydrodiol. High performance liquid chromatographic analysis of the metabolites was used to determine the ratio of metabolites to unmetabolized parent compound and then compared with control samples. Three of the ten compounds affected epoxide hydratase activity. Capsaicin inhibited; whereas, catechin and esculetin enhanced EH activity. Seven of the compounds tested inhibited microsomal mixed‐function oxidase activity. In order of potency, these seven compounds were tannic acid, d‐limonene, capsaicin, ellagic acid, propyl gallate, esculetin and catechin. Since the mutagenic and/or carcinogenic action of many chemicals is dependent upon the metabolic activity of microsomal mixed‐function oxidase and epoxide hydrase, the modulation of this activity by these chemicals provides further evidence in support of their potential chemopreventive properties.