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Tissue cultures of Withania somnifera : Morphogenesis and withanolide synthesis
Author(s) -
Roja G.,
Heble M. R.,
Sipahimalani A. T.
Publication year - 1991
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650050411
Subject(s) - withania somnifera , callus , withanolide , explant culture , biology , botany , shoot , tissue culture , murashige and skoog medium , morphogenesis , auxin , cytokinin , meristem , traditional medicine , in vitro , biochemistry , medicine , alternative medicine , pathology , gene
Tissue cultures were established from axillary meristems of the plant Withania somnifera . Growth hormones influenced the morphogenetic responses of the cultures. The explants gave multiple shoots in Murashige and Skoog's ( MS ) medium supplemented with benzyladenine (BA), rooting in MS +coconut milk (CM) (10%) and callus formation in MS+2,4‐dichlorophenoxyacetic acid (2,4‐D) (2.0 ppm). Callus cultures failed to synthesize withanolides. Multiple shoot cultures synthesized significant levels of withanolides and their concentrations varied with different growth hormones.