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Anoectochilus formosanus Hay. Contains substances that affect arachidonic acid metabolism
Author(s) -
Mak OiTong,
Huang DinqDing,
Law Rocky ChauSing
Publication year - 1990
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650040202
Subject(s) - hay , incubation , ethyl acetate , platelet , biology , biochemistry , arachidonic acid , chemistry , prostacyclin , botany , enzyme , immunology
The whole plant of Anoectochilus formosanus Hay. was extracted with hot water, and the aqueous solution was re‐extracted with ethyl acetate in order to study the effects on thromboxane A 2 (TXA 2 ) production in rabbit platelets and prostacyclin (PGI 2 ) production in bovine aortic tissues. Inhibition of TXA 2 production, using either arachidonic acid (AA) or PGH 2 as precursors, was manifested on incubation of platelets with the crude water extraction, and the ethyl acetate extraction of A. formosanus Hay., respectively; the inhibitory effects were concentration‐dependent. Incubation of platelets with the aqueous remainder caused activation of TXA 2 production, using AA as the precursor, but there was no effect on TXA 2 production using PGH 2 as the precursor. With the AA precursor, there was activation of PGI 2 production on incubation of aortic tissues with both crude water extraction and aqueous remainder of A. formosanus Hay., but not with ethyl acetate extraction, and the activating effects were also concentration‐dependent. No effect on PGI 2 production was observed on incubation with PGH 2 as the precursor. These results suggest that A. formosanus Hay. contains at least two different components affecting TXA 2 production in platelets, and PGI 2 production in aortic endothelium, respectively, and these effects might play important roles in the pharmacological effects of A. formosanus Hay.