Premium
Inhibition of cell growth and macromolecule biosynthesis of human promyelocytic leukemic cells by acridone alkaloids
Author(s) -
Chou TingChao,
Tzeng CherngChyi,
Wu TianShung,
Watanabe Kyoichi A.,
Su TsannLong
Publication year - 1989
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650030604
Subject(s) - acridone , trypan blue , cytotoxicity , macromolecule , biochemistry , cell growth , dna , biosynthesis , chemistry , cell culture , cell , growth inhibition , rna , dna synthesis , biology , microbiology and biotechnology , stereochemistry , in vitro , enzyme , gene , genetics
Acridone alkaloids of plants indigenous to the Orient were isolated and purified, and their chemical structures were determined. A total of 50 acridones and their synthetic derivatives were subjected to rapid screening for inhibition of macromolecular synthesis using radiolabeled precursors of DNA, RNA and protein. These compounds were then studied to examine cell growth inhibition of human promyelocytic leukemic cells (HL‐60). Cytotoxicity was studied using the trypan blue exclusion method. Of 50 compounds evaluated for cell growth inhibition, 12 compounds showed IC 50 <10 μM; 12 compounds, 10‐20 μM; 8 compounds, 20‐100 μM; and 18 compounds, >100 μM.