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Pharmacological actions of the gut active principles in Croton penduliflorus hutch. Seed on isolated smooth and skeletal muscles and on blood pressure
Author(s) -
Asuzu I. U.,
Shetty S. N.,
Anika S. M.
Publication year - 1988
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650020404
Subject(s) - ileum , mepyramine , hexamethonium , atropine , guinea pig , muscle contraction , histamine , chemistry , pharmacology , anatomy , anesthesia , biology , endocrinology , medicine , biochemistry , antagonist , receptor
The effects of Croton penduliflorus seed crystals (CP crystals) were investigated on isolated guinea‐pig ileum, chick biventer cervicis muscle and frog rectus abdominis muscle. The effect of the crystals on flood pressure of normotensive Sprague Dawley rats was also investigated. CP crystals induced concentrations‐dependent contractions in the guinea‐pig ileum with EC 50 of 2.39 × 10 6 g/mL. Contractions induced by CP crystals in the guinea‐pig ileum were not inhibited by atropine (2 × 10 −7 ‐1.2 × 10 −6 g/mL), tetrodotoxin (3 × 10 −8 ‐2 × 10 −7 g/mL), hexamethonium (7 × 10 −6 ‐2.8 × 10 −5 g/mL), mepyramine (2 × 10 −7 g/mL) and noradrenaline (2 × 10 −5 g/mL) but were completely (100%) inhibited by indomethacin (7.2 × 10 −6 g/mL). CP crystals (1.0 × 10 −6 ‐5.0 × 10 −4 g/mL) could not elicit contractions in either chick biventer cervicis or frog rectus abdominis muscle. The mean blood pressure of normotensive Sprague Dawley rats was significantly reduced by CP crystals (1.6 × 10 −4 ‐2.5 × 10 −3 g/mL) while the heart rate was significantly reduced by an intravenous infusion of the crystals (2.5 × 10 −3 g/kg) after 2min. The methods employed and the significance of the results obtained are discussed.