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Effects of chalcones isolated from licorice roots on leukotriene biosynthesis in human polymorphonuclear neutrophls
Author(s) -
Kimura Yoshiyuki,
Okuda Hiromichi,
Okuda Takuo,
Arichi Shigeru
Publication year - 1988
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650020308
Subject(s) - ionophore , chemistry , calcium , leukotriene , glycyrrhiza , egta , cytosol , chalcone , biochemistry , pharmacology , stereochemistry , biology , immunology , medicine , organic chemistry , enzyme , alternative medicine , pathology , asthma
Abstract The effects of chalcones and flavanones isolated from Sinkiang Licorice ( Glycyrrhiza inflata Bat.) on leukotriene B 4 (LTB 4 ) and C 4 (LTC 4 ) formation in human polymorphonuclear neutrophils (PMNs) were investigated. Licochalcone A (2‐methoxy‐4,4′‐dihydroxy‐5‐α,α‐dimethyl‐allylchalcone) and licochalcone B (2‐methoxy‐3,4,4′‐trihydroxychalcone), at concentrations of 10 −3 to 10 −6 M, inhibited calcium ionophore A 23187 (A 23187)‐induced LTB 4 and LTC 4 formation in human PMNs. The concentration of licochalcones A required for 50% inhibition (LC 50 ) of LTB 4 and LTC 4 formation was 4.6 × 10 −7 and 4.2×10 −6 M, respectively, using 2 μM A 23187. The IC 50 values of licochalcone B for LTB 4 and LTC 4 formation were also 1.2 × 10 −6 and 2.0×10 −6 M, respectively. In addition, licochalcones A and B significantly increased cyclic AMP level in human PMNs at concentrations of 10 −4 to 10 −3 M. Licochalcones A and B were found to reduce the elevation of the cytosolic free calcium concentration induced by calcium ionomycine, dose‐dependently, in the presence of 1 mM Ca 2+ or 1 mM EGTA.