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In vitro cytotoxicity testing of natural products using guinea‐pig ear keratinocytes
Author(s) -
Harris Ann,
Keene Alison T.,
Phillipson J. David,
Warhurst David C.
Publication year - 1988
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2650020103
Subject(s) - guinea pig , in vitro , cytotoxicity , pharmacology , colchicine , ajmaline , pharmacognosy , berberine , vincristine , emetine , apocynaceae , alkaloid , quinine , biology , traditional medicine , chemistry , biochemistry , medicine , biological activity , stereochemistry , immunology , chemotherapy , endocrinology , neuroscience , genetics , malaria , cyclophosphamide
Abstract A simple test is described in which the inhibition of uptake of [G‐ 3 H]thymidine into guinea‐pig ear keratinocytes in vitro , has been used as an assessment for cytotoxicity of a series of alkaloids. The alkaloids tested include emetine, colchicine, coronaridine, ajmaline, aricine, vincristine, leurosine, quinamine, 3‐epiquinamine, quinine, quinidinone, cichonidine, berberine and atropine.