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Tanacetum vulgare : antiherpes virus activity of crude extract and the purified compound parthenolide
Author(s) -
Onozato Thelma,
Nakamura Celso Vataru,
Cortez Diógenes Aparício Garcia,
Filho Benedito Prado Dias,
UedaNakamura Tânia
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2638
Subject(s) - parthenolide , vero cell , herpes simplex virus , virus , cell culture , asteraceae , pharmacognosy , in vitro , biology , chemistry , virology , traditional medicine , biological activity , biochemistry , medicine , botany , apoptosis , genetics
The present study demonstrated that the ethyl acetate extract and the isolated compound, parthenolide, from aerial parts of Tanacetum vulgare L. (Asteraceae), protected Vero cells from herpes simplex virus (HSV‐1) infection in vitro . The extract and parthenolide were assayed against HSV‐1 by the sulforhodamine B colorimetric assay and exhibited anti‐HSV‐1 activity with an EC 50 of 40 µg/mL and 0.3 µg/mL, respectively. In order to determine which stage of the virus–cell interaction was affected by parthenolide, the pure compound was used. No effect was observed when both viruses and cells were pretreated, or during early stages of infection, suggesting that parthenolide interfered with virus replication after the penetration stage, inhibiting approximately 40% of plaques formed at a concentration of 2.5 µg/mL when compared with an untreated control. Copyright © 2009 John Wiley & Sons, Ltd.