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Cytotoxic activity of alkaloids isolated from Stephania rotunda in vitro cytotoxic activity of cepharanthine
Author(s) -
Bun SokSiya,
Laget Michèle,
Chea Aun,
Bun Hot,
Ollivier Evelyne,
Elias Riad
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2617
Subject(s) - tetrahydropalmatine , cytotoxicity , menispermaceae , cytotoxic t cell , mtt assay , in vitro , alkaloid , traditional medicine , pharmacology , microsome , chemistry , biology , biochemistry , stereochemistry , medicine , corydalis , pathology , alternative medicine , traditional chinese medicine
Three major alkaloids: cepharanthine (1), tetrahydropalmatine (2) and xylopinine (3) isolated from Stephania rotunda tuber were investigated for their cytotoxic activity in a panel of human cancer cells (HT29, LS174T, SW620 and HepG2) using MTT assay. In the present study, cepharanthine (1) exerted potent cytotoxicity against colon and hepatoma cancer cell lines with IC 50 values between 2.4 and 5.3 µM while tetrahydropalmatine (2) and xylopinine (3) displayed weak cytotoxicity. In addition, the mutagenic activity of cepharanthine (1) was investigated using a modified liquid incubation technique of the Salmonella/microsomal assay. This alkaloid (1) was found to be non‐mutagenic for doses up to 8.2 µM. Copyright © 2008 John Wiley & Sons, Ltd.