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Extracts of Cynomorium songaricum protect SK‐N‐SH human neuroblastoma cells against staurosporine‐induced apoptosis potentially through their radical scavenging activity
Author(s) -
Lu Yi,
Wang Qingguo,
Melzig Matthias F.,
JenettSiems Kristina
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2605
Subject(s) - staurosporine , xanthine oxidase , apoptosis , pharmacology , traditional medicine , programmed cell death , superoxide , xanthine , chemistry , antioxidant , biochemistry , medicine , enzyme , protein kinase c
In traditional Chinese medicine a number of herbs are used to alleviate the symptoms of aging, among them the stems of Cynomorium songaricum , Cynomoriaceae. This study evaluated the protective effect of different extracts of C. songaricum on staurosporine‐induced apoptotic cell death in SK‐N‐SH neuroblastoma cells. Staurosporine (100 n m ) reduced cell viability to about 55%. The ethyl acetate fraction of C. songaricum significantly attenuated staurosporine‐induced cell death at concentrations of 100 and 10 µg/mL. On the other hand, the dichloromethane as well as water fractions showed no protective effects. In order to further analyse the protective mode of action, the superoxide anion scavenging activity of two extracts was evaluated in a xanthine/xanthine oxidase system. In this system, the EtOAc extract showed a good scavenging activity (IC 50 value 2.9 µg/mL) without inhibition of xanthine oxidase. In conclusion, the results prove the neuroprotective activity of C. songaricum extracts in vitro , thus supporting its traditional use. Copyright © 2008 John Wiley & Sons, Ltd.