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Genotoxicity of Achillea millefolium essential oil in diploid cells of Aspergillus nidulans
Author(s) -
Sant'Anna Juliane Rocha de,
Franco Claudinéia Conationi da Silva,
Miyamoto Claudia Tiemi,
Cunico Miriam Machado,
Miguel Obdulio Gomes,
Côcco Lílian Cristina,
Yamamoto Carlos Itsuo,
Junior Cirino Corrêa,
CastroPrado Marialba Avezum Alves de
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2596
Subject(s) - achillea millefolium , genotoxicity , sabinene , essential oil , biology , eucalyptol , botany , traditional medicine , microbiology and biotechnology , chemistry , toxicity , medicine , organic chemistry , limonene
The essential oil of Achillea millefolium is commonly used in folk medicine for the treatment of several diseases and has been demonstrated previously to exert an in vitro antimicrobial activity against human pathogens. Current study investigates the genotoxic activity of A. millefolium oil. The oil's major constituents are: chamazulene (42.15%), sabinene (19.72%), terpin‐4‐ol (5.22%), β ‐caryophyllene (4.44%) and eucalyptol (3.10%), comprising 74.63% of the total. The oil's genotoxic evaluation was performed at concentrations of 0.13 µL/mL, 0.19 µL/mL and 0.25 µL/mL with a heterozygous diploid strain of Aspergillus nidulans , named A757//UT448, with green conidia. A statistically significant increasing number of yellow and white mitotic recombinants, per colony, of the diploid strain was reported after oil treatment with 0.19 µL/mL and 0.25 µL/mL concentrations. The genotoxicity of the oil was associated with the induction of mitotic non‐disjunction or crossing‐over by oil. Copyright © 2008 John Wiley & Sons, Ltd.