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Optimization of extraction and purification of active fractions from Schisandra chinensis (Turcz.) and its osteoblastic proliferation stimulating activity
Author(s) -
Caichompoo Wanida,
Zhang QiaoYan,
Hou TingTing,
Gao HuaJuan,
Qin LuPing,
Zhou XiuJia
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2585
Subject(s) - schisandra chinensis , ethanol , chemistry , chromatography , extraction (chemistry) , alkaline phosphatase , high performance liquid chromatography , lignan , in vitro , enzyme , biochemistry , stereochemistry , medicine , alternative medicine , pathology , traditional chinese medicine
Extraction and purification conditions of lignans from the fruits and seeds of Schisandra chinensis (Turcz.) were investigated through an orthogonal design of L 9 (3 4 ) assay and macroporous resin technology. The extraction was optimized using 95% ethanol. For purification, the extract was dissolved in 30% ethanol, then adsorbed on a AB‐8 macroporous resin and eluted with 30% ethanol and 70% ethanol successively, the latter resulting in a residue containing 65.2% of lignans. By HPLC analysis schisandrin, deoxyschisandrin and γ ‐schisandrin were quantitatively determined. UMR 106 cells were used to examine the stimulatory activity of the lignans on osteoblasts in vitro . The lignans stimulated the proliferation of and the activity of alkaline phosphatase in the osteoblasts indicating their potential activity against osteoporosis. Copyright © 2008 John Wiley & Sons, Ltd.