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The isoflavones mixture from Trifolium pratense L. protects HCN 1‐A neurons from oxidative stress
Author(s) -
Occhiuto Francesco,
Palumbo Dora Rita,
Samperi Stefania,
Zangla Giuseppe,
Pino Annalisa,
Pasquale Rita De,
Circosta Clara
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2584
Subject(s) - isoflavones , biochanin a , genistein , formononetin , neuroprotection , daidzein , oxidative stress , viability assay , programmed cell death , antioxidant , trypan blue , biochemistry , chemistry , pharmacology , biology , endocrinology , cell , apoptosis
Oxidative stress‐induced neuronal cell death has been implicated in different neurological disorders and neurodegenerative diseases such as Alzheimer's disease and Parkinson's. Using the Alzheimer's disease‐associated hydrogen peroxide (H 2 O 2 ), we investigated the neuroprotective efficacy of a natural mixture of phytoestrogenic isoflavones (genistein, daidzein, biochanin A and formononetin) from Trifolium pratense L. (Red clover) against oxidative stress‐induced cell death in human cortical cell line HCN 1‐A maintained in culture. Neuronal viability was determined by MTT or trypan blue test and neuronal integrity by morphological analysis. The results obtained indicate that exposure of HCN 1‐A cell cultures to hydrogen peroxide resulted in a concentration‐dependent decrease in neuron viability. Concentration of H 2 O 2 ranging from 50 to 200 µg/ml were toxic to these cultures. A 24‐hour pretreatment with 0.5, 1 and 2 µg/ml isoflavones extract significantly increased cell survival as evidenced by MTT or trypan blue test and significantly prevented the morphological disruption caused by H 2 O 2 as shown by microscopical inspection, indicating that neurons treated with isoflavones were protected from the cell death induced by H 2 O 2 exposure. These findings imply that the neuroprotective effect of isoflavones extract is partly associated with its antioxidant activity. Further, results of these investigations indicate that although isoflavones extract exert a neuroprotective effect, it do not promoted cortical neuron process outgrowth. Copyright © 2008 John Wiley & Sons, Ltd.