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Gallotannin ameliorates the development of streptozotocin‐induced diabetic nephropathy by preventing the activation of PARP
Author(s) -
Chandak Prakash Gopaldas,
Gaikwad Anil Bhanudas,
Tikoo Kulbhushan
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2559
Subject(s) - poly adp ribose polymerase , diabetic nephropathy , oxidative stress , streptozotocin , diabetes mellitus , apoptosis , endocrinology , medicine , nephropathy , parp inhibitor , pharmacology , chemistry , biology , biochemistry , enzyme , polymerase
Abstract Poly(ADP‐ribose) polymerase (PARP) is known to be activated under conditions of oxidative stress and/or radiation exposure. The role of this enzyme has been well demonstrated in the streptozotocin (STZ) induced model of diabetes. Inhibition of PARP by specific inhibitors is known to prevent the development of STZ induced diabetic nephropathy by reduction in oxidative stress induced apoptosis. This study shows for the first time the role of poly(ADP‐ribose) glycohydrolase (PARG) inhibitors as an alternative approach for inhibition of PARP. Gallotannin (20 mg/kg/day, i.p.) treatment for 4 weeks led to a significant reduction in the levels of plasma creatinine which is a well known marker for diabetic nephropathy. Treatment with gallotannin resulted in protection up to a certain level of glomerular damage, suggesting compensatory glomerular hypertrophy. As a PARG inhibitor gallotannin treatment also showed protection in PARP cleavage which is a hallmark for apoptotic cell death signifying the protective role of gallotannin in cell death signaling. Copyright © 2008 John Wiley & Sons, Ltd.