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Determination of ginsenoside‐Rg 1 in human plasma and its application to pharmacokinetic studies following intravenous administration of ‘Shenmai’ injection
Author(s) -
Yang Liu,
Xu Shunjun,
Wu Zhifeng,
Liu YiMing,
Zeng Xing
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2558
Subject(s) - pharmacokinetics , chromatography , chemistry , ginseng , electrospray ionization , selected reaction monitoring , ginsenoside , extraction (chemistry) , analyte , mass spectrometry , radix (gastropod) , solid phase extraction , pharmacology , tandem mass spectrometry , medicine , biology , botany , alternative medicine , pathology
‘Shenmai’ injection is derived from traditional Chinese medicine ‘Shenmaisan’ and made from Radix ginseng Rubra and Radix Ophiopogonis. Ginsenoside‐Rg 1 , as a major constituent of Radix ginseng Rubra, is considered responsible for the efficacy of this injection. A rapid, simple and accurate method has been established for determination of ginsenoside‐Rg 1 in Shenmai injection and human plasma using LC‐ESI‐MS/MS, and to study the pharmacokinetics of Rg 1 in ten healthy volunteers after intravenous single dosing of 60 mL of Shenmai injection. Following solid‐phase extraction (SPE), samples were separated on a C 18 column coupled with electrospray ionization mass spectrometry. The protonated analyte was quantified by multiple reaction monitoring (MRM) with a quadruple mass spectrometer in positive mode. Linearity was confirmed in the concentration range of 1 to 1000 ng/mL for Rg 1 , and the lower limit of quantification (LLOQ, S/N > 10) was 1 ng/mL. The intraday and interday RSDs were within 15% and mean extraction recoveries ranged from 98.6% to 104.9%. The pharmacokinetics of Rg 1 in healthy volunteers conforms to the two‐compartment open model. The main pharmacokinetics parameters were as follows: t 1/2 β , 2.09 ± 1.89 h; CL, 0.03 ± 0.01 L kg −1 h −1 ; AUC (0 ∼ ∞), 124.4 ± 35.9 2 ng mL −1 h and AUC (0 ∼ ∞), 127.9 ± 37.2 ng mL −1 h, respectively. Copyright © 2008 John Wiley & Sons, Ltd.