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Buddleja officinalis inhibits high glucose‐induced matrix metalloproteinase activity in human umbilical vein endothelial cells
Author(s) -
Lee Yun Jung,
Kang Dae Gill,
Kim Jin Sook,
Lee Ho Sub
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2547
Subject(s) - umbilical vein , matrix metalloproteinase , oxidative stress , endocrinology , abo blood group system , medicine , metalloproteinase , officinalis , biology , immunology , biochemistry , traditional medicine , in vitro
The aim of the present investigation was to investigate whether an aqueous extract of Buddleja officinalis (ABO), a traditional Korean herbal medicine, suppresses the endothelial extracellular matrix degradation under high glucose condition. The incubation with high concentration of glucose (25 mM) increased significantly matrix metalloproteinase (MMP)‐2/‐9 expressions and activities in primary cultured human umbilical vein endothelial cells (HUVEC). Pretreatment with ABO decreased high glucose‐induced increase of MMP‐2/‐9 activities in a dose‐dependent manner. Real time qRT‐PCR revealed that high glucose‐induced MMP‐2/‐9 mRNA expression levels were attenuated by pretreatment with ABO. High glucose‐induced MCP‐1 and IL‐8 mRNA expression levels also decreased by ABO. ABO decreased high glucose‐induced hydrogen peroxide production, oxidative stress marker. These results provide new insights into the pathophysiological mechanisms for anti‐inflammatory properties of ABO in vascular diseases associated with diabetes mellitus. Copyright © 2008 John Wiley & Sons, Ltd.