Activity‐guided isolation of a novel protein from  Croton tiglium  with antifungal and antibacterial activities | Zendy

Shahid Muhammad | Zendy; Tayyab Muhammad | Zendy; Naz Farah | Zendy; Jamil Amer | Zendy; Ashraf Muhammad | Zendy; Gilani Anwarul Hassan | Zendy

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Activity‐guided isolation of a novel protein from Croton tiglium with antifungal and antibacterial activities

Author(s) -

Shahid Muhammad,

Tayyab Muhammad,

Naz Farah,

Jamil Amer,

Ashraf Muhammad,

Gilani Anwarul Hassan

Publication year - 2008

Publication title -

phytotherapy research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 129

eISSN - 1099-1573

pISSN - 0951-418X

DOI - 10.1002/ptr.2544

Subject(s) - antimicrobial , chemistry , polyacrylamide gel electrophoresis , antibacterial activity , croton , size exclusion chromatography , chromatography , antifungal , ion chromatography , molecular mass , traditional medicine , biochemistry , bacteria , microbiology and biotechnology , biology , enzyme , organic chemistry , medicine , genetics

This study describes the activity‐guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH 4 ) 2 SO 4 precipitation, gel filtration and DEAE‐cellulose ion‐exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS‐polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium , which possesses a strong and broad spectrum antimicrobial activity. Copyright © 2008 John Wiley & Sons, Ltd.

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