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Antiinflammatory effect of the ethanol extract of Berberis koreana in a gerbil model of cerebral ischemia/reperfusion
Author(s) -
Yoo KiYeon,
Hwang In Koo,
Kim Jong Dai,
Kang IlJun,
Park Jinseu,
Yi JaeSeon,
Kim JinKyu,
Bae YoungSoo,
Won MooHo
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2527
Subject(s) - gerbil , neuroprotection , ischemia , pharmacology , medicine , in vivo , prostaglandin e2 , hippocampal formation , biology , microbiology and biotechnology
Berberis koreana extract (BE) has a strong neuroprotective effect after ischemic stroke in gerbils, which is associated with the inhibition of the N ‐methyl‐d‐aspartate receptor. The present study examined the antiinflammatory mechanism of BE after ischemic damage in vitro and in vivo . The BE used contained on average 7.39 ± 0.78 mg/g of berberine. In PC12 cells with inflammation, prostaglandin E2 (PGE2) production was significantly reduced by BE. About 75% of pyramidal cells in the hippocampal CA1 region of gerbils exposed to 5 min of transient ischemia were protected from ischemic damage by BE. Cyclooxygenase‐2 (COX‐2) immunoreactivity and its protein level in the CA1 region of vehicle‐treated animals exposed to an ischemic insult increased with time post‐ischemia, whereas no such changes were observed in BE‐treated animals exposed to ischemia. PGE2 production in BE‐treated ischemic animals was significantly lower than that observed in vehicle‐treated ischemic animals. Summarizing, the potent neuroprotective effect of BE was found to be due to the inhibitions of COX‐2 expression and PGE2 production and its antiinflammatory activity. Copyright © 2008 John Wiley & Sons, Ltd.