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Evaluation of antioxidant, antinociceptive, and anti‐inflammatory activities of ethanol extracts from Aloe saponaria Haw.
Author(s) -
Yoo Eun Ah,
Kim Sung Dae,
Lee Whi Min,
Park Hwa Jin,
Kim Sang Keun,
Cho Jae Youl,
Min Wongi,
Rhee Man Hee
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2514
Subject(s) - chemistry , antioxidant , xanthine oxidase , pharmacology , ethanol , reactive oxygen species , dpph , nitric oxide synthase , lipopolysaccharide , superoxide dismutase , biochemistry , traditional medicine , enzyme , medicine , immunology
Aloe species are traditionally prescribed for hypertension, burning, and rheumatoid arthritis. To elucidate the mechanism of the antihypertensive and anti‐inflammatory activities of this herb, the ethanol fraction from A. saponaria Haw. was evaluated for antioxidative activity using xanthine‐xanthine oxidase (XO) assay, 2,2‐Diphenyl‐lpicrylhydrazyl radical (DPPH) assay, lipopolysaccharide (LPS)‐induced nitric oxide (NO) production in RAW 264.7 cell, and antinociceptive activity using a tail‐flick assay and hind paw pressure assay in cisplatin‐treated hyperalgesic rats. The ethanol fraction displayed potent antioxidative activities in XO assay. In addition, ethanol fractions showed potent scavenging effects in DPPH assay. We next examined whether ethanol fractions showed anti‐inflammatory activities. Ethanol fractions significantly suppressed NO production from LPS‐activated RAW264.7 cells. As expected, ethanol fractions dose‐dependently inhibited the messenger RNA expression of inducible NO synthase (iNOS). Moreover, ethanol fractions potently suppressed the expression of cycloxygenase (COX)‐2 and granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), which are stimulated by LPS in RAW264.7 cells. In addition, ethanol fractions significantly blocked cisplatin‐induced hyperalgesia using tail‐flick assay and hind paw pressure test in rats. Taken altogether, ethanol extracts of aloe may be useful as a functional food or as a drug against reactive oxygen species (ROS) mediated diseases. Copyright © 2008 John Wiley & Sons, Ltd.