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Isolation of linoleic and α ‐linolenic acids as COX‐1 and ‐2 inhibitors in rose hip
Author(s) -
Jäger A. K.,
Petersen K. N.,
Thomasen G.,
Christensen S. Brøgger
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2446
Subject(s) - linoleic acid , linolenic acid , chemistry , fractionation , pharmacognosy , bioassay , chromatography , oleic acid , food science , biochemistry , fatty acid , biological activity , biology , in vitro , genetics
Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase‐1 and ‐2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C‐18 column and by HPLC. Each step was COX‐1/2 activity‐guided. The bioassay‐guided fractionation led to the isolation of linoleic acid (the IC 50 for COX‐1 was 85 µ m and 0.6 µ m for COX‐2) and α ‐linolenic acid (the IC 50 for COX‐1 was 52 µ m and 12 µ m for COX‐2). The COX‐2/COX‐1 ratio was 0.007 for linoleic acid and 0.2 for α ‐linolenic acid. Linoleic acid and α ‐linolenic acid contribute to the COX‐1 and ‐2 inhibitory activity of rose hip. Copyright © 2008 John Wiley & Sons, Ltd.