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Wen‐pi‐tang‐Hab‐Wu‐ling‐san attenuates kidney fibrosis induced by ischemia/reperfusion in mice
Author(s) -
Seok Young Mi,
Kim Jinu,
Park Mae Ja,
Boo Yong Chool,
Park YongKi,
Park Kwon Moo
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2440
Subject(s) - kidney , medicine , superoxide dismutase , fibrosis , ischemia , pharmacology , lipid peroxidation , oxidative stress , endocrinology
Renal fibrosis is highly implicated as a cause of chronic renal failure, for which suitable therapeutics have not yet been developed. Recently, it was reported that Wen‐pi‐tang‐Hab‐Wu‐ling‐san (WHW) extract attenuates epithelial cells undergoing mesenchymal transition in cultured Madin‐Darby canine kidney cells. This study investigated whether WHW extract prevents renal fibrosis induced by ischemia/reperfusion (I/R) in mice. Ischemia/reperfusion resulted in kidney fibrosis at 14 days after the procedure. When WHW was administered orally to mice beginning from 2 days after the onset of ischemia until killing, the fibrosis was significantly reduced. WHW administration significantly prevented a post‐ischemic decrease of copper‐zinc superoxide dismutase (CuZnSOD) and manganese superoxide dismutase (MnSOD) activities, leading to decreased lipid peroxidation and hydrogen peroxide production. In addition, WHW administration attenuated the phosphorylation of extracellular signal‐regulated kinase 1/2 (ERK1/2) and c‐Jun N ‐terminal kinase 1/2 (JNK1/2) and attenuated the activation of nuclear factor‐kappa B (NF‐ κ B) in the kidneys subjected to ischemia. In conclusion, WHW extract attenuated the renal fibrosis and the attenuation was associated with a reduction of oxidative stress and an inhibition of ERK1/2, JNK1/2, p38 and NF‐ κ B activation. WHW extract may be an attractive agent to attenuate the progression of fibrosis. Copyright © 2008 John Wiley & Sons, Ltd.