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Astragaloside IV attenuates lipolysis and improves insulin resistance induced by TNF α in 3T3‐L1 adipocytes
Author(s) -
Jiang Boren,
Yang Ying,
Jin Hua,
Shang Wenbin,
Zhou Libin,
Qian Lei,
Chen Mingdao
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2434
Subject(s) - lipolysis , insulin resistance , endocrinology , medicine , 3t3 l1 , adipocyte , insulin , tumor necrosis factor alpha , pharmacology , chemistry , adipose tissue
Increased circulating free fatty acid (FFA) concentrations have been demonstrated to potentially link obesity, insulin resistance and cardiovascular diseases. Astragaloside IV (AS‐IV) is a saponin which is widely used in traditional Chinese medicine to treat type 2 diabetes and cardiovascular diseases. The purpose of the present study was to examine the effects of AS‐IV on the lipolysis and insulin resistance induced by tumor necrosis factor‐ α (TNF α ) in cultured 3T3‐L1 adipocytes. TNF α promotes lipolysis in mammal adipocytes via the mitogen activated protein kinase (MAPK) family resulting in reduced expression/function of perilipin. Application of AS‐IV inhibited TNF α ‐induced accelerated lipolysis in a dose‐dependent manner, which was compatible with suppressed phosphorylation of ERK1/2 and reversed the downregulation of perilipin. Moreover, TNF α induced downregulation of key enzymes in lipogenesis, including LPL, FAS and GPAT, were also attenuated by AS‐IV. Further studies showed that AS‐IV improved TNF α ‐induced insulin resistance in 3T3‐L1 adipocytes. This study provides the first direct evidence of the antilipolytic action of AS‐IV in adipocytes, which may allow this agent to decrease the circulating FFA levels, thus increase insulin sensitivity and treat cardiovascular diseases. Copyright © 2008 John Wiley & Sons, Ltd.