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Isolation and identification of cytotoxic compounds from the wood of Pinus resinosa
Author(s) -
Simard François,
Legault Jean,
Lavoie Serge,
Mshvildadze Vakhtang,
Pichette André
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2416
Subject(s) - cytotoxicity , chemistry , a549 cell , cytotoxic t cell , cell culture , stereochemistry , dimethyl ether , biochemistry , biology , cell , in vitro , genetics , catalysis
Methanol extracts of wood from Pinus resinosa were found to be selectively cytotoxic against human lung carcinoma cells, A549 (IC 50 41 ± 6 µg/mL), human colorectal adenocarcinoma cells, DLD‐1 (IC 50 47 ± 4 µg/mL) in comparison with healthy cells, WS1 (IC 50 130 ± 11 µg/mL). Five known compounds were isolated and identified by 1 H, 13 C NMR spectroscopy and HR‐ESI‐MS mass spectrometry as, pinosylvin monomethyl ether (1), pinosylvin (2), pinosylvin dimethyl ether (3), pinobanksin (4) and (‐)‐norachelogenin (5). Compound 4 was isolated for the first time in P. resinosa . The cytotoxicity of compounds 1–5 was evaluated against A549, DLD‐1 and WS1. Compound 1 exhibited the strongest cytotoxicity against both tumor cell lines and the healthy cell line with an IC 50 of 25 ± 4 µ m for A549, 20 ± 1 µ m for DLD‐1 and 34 ± 3 µ m for WS1. Copyright © 2008 John Wiley & Sons, Ltd.