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Inhibitory effects of the aqueous extract of Magnolia officinalis on the responses of human urinary bladder cancer 5637 cells in vitro and mouse urinary bladder tumors induced by N ‐Butyl‐ N ‐(4‐hydroxybutyl) nitrosamine in vivo
Author(s) -
Lee SeJung,
Cho YoungHwa,
Park Keerang,
Kim EunJung,
Kang BongSu,
Jung KyungHwan,
Kim CheorlHo,
Kim WunJae,
Moon SungKwon
Publication year - 2009
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2413
Subject(s) - magnolia officinalis , officinalis , apoptosis , in vivo , urinary bladder , in vitro , urinary system , chemistry , bladder cancer , nitrosamine , cancer research , carcinogen , pharmacology , cancer , biology , medicine , pathology , biochemistry , endocrinology , botany , alternative medicine , microbiology and biotechnology , traditional chinese medicine
This study investigated the anticancer activity of Magnolia officinalis on urinary bladder cancer in vitro and in vivo , and elucidated the mechanism of its activity. An aqueous extract of M. officinalis inhibited cell viability and DNA synthesis in cultured human urinary bladder cancer 5637 cells. Inhibition of proliferation was the result of apoptotic induction, because FACS analyses of 5637 cells treated with M. officinalis showed a sub‐G1 phase accumulation. M. officinalis extract also increased cytoplasmic DNA–histone complex dose‐dependently. These inhibitory effects were associated with the upregulation of proapoptotic molecules Bax, cytochrome c and caspase 3. Treatment of 5637 cells with M. officinalis extract suppressed the expression of matrix metalloproteinase 2 (MMP‐2) and MMP‐9, as revealed by zymographic and immunoblot analyses. When M. officinalis extract was given to mice simultaneously with the carcinogen N ‐butyl‐ N ‐(4‐hydroxybutyl) nitrosamine, which induces urinary bladder tumors, the size of the induced tumors was smaller. Finally, histological data indicated that the histological grade of carcinoma and the depth of invasion were dramatically decreased by treatment with M. officinalis extract in mice with N ‐butyl‐ N ‐(4‐hydroxybutyl) nitrosamine‐induced urinary bladder tumors. In conclusion, the findings showed that M. officinalis extract exhibited potential chemopreventive activity against urinary bladder tumor in vitro and in vivo . Copyright © 2008 John Wiley & Sons, Ltd.