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Inhibitory effects of Angelicae Gigantis Radix on osteoclast formation
Author(s) -
Kil JongSeob,
Kim MyungGyou,
Choi Hye Mi,
Lim JongPil,
Boo Yungmin,
Kim EeHwa,
Kim JeungBeum,
Kim Hye Kyung,
Leem KangHyun
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2342
Subject(s) - osteoclast , bone marrow , radix (gastropod) , acid phosphatase , tartrate resistant acid phosphatase , multinucleate , chemistry , tumor necrosis factor alpha , inhibitory postsynaptic potential , microbiology and biotechnology , biochemistry , immunology , biology , medicine , in vitro , enzyme , botany
Angelicae Gigantis Radix (AGR) is one of the most widely used herbal medications. AGR is the dried root of Angelica gigas Nakai (Umbelliferae), which is known as Korean angelica. This study investigated the effects of AGR on osteoclast formation using primary bone marrow cells. TNF‐ α treatment increased tartrate‐resistant acid phosphatase (Trap) positive cells and Trap activity in bone marrow cells. However, AGR significantly decreased both TNF‐ α ‐induced Trap positive cells and Trap activity. RT‐PCR analyses revealed that AGR decreased mRNA levels of Trap and matrix metalloproteinase‐9 in TNF‐ α ‐treated bone marrow cells. In addition, AGR decreased TNF‐ α ‐induced activation of NF‐ κ B. These results suggest that AGR has an inhibitory effect on the formation of osteoclasts and its effect is partially related to the NF‐ κ B pathway. Copyright © 2008 John Wiley & Sons, Ltd.