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Acanthopanax senticosus suppresses reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo
Author(s) -
Lin QiuYe,
Jin LiJi,
Cao ZhenHui,
Lu YaNan,
Xue HongYu,
Xu YongPing
Publication year - 2008
Publication title -
phytotherapy research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 129
eISSN - 1099-1573
pISSN - 0951-418X
DOI - 10.1002/ptr.2341
Subject(s) - superoxide , zymosan , reactive oxygen species , in vivo , hydrogen peroxide , ex vivo , in vitro , chemistry , pharmacology , phorbol , biochemistry , biology , signal transduction , enzyme , protein kinase c , microbiology and biotechnology
Abstract Excess production of reactive oxygen species by macrophages has been implicated in many inflammatory diseases. The present study investigated the inhibitory effect of the stem bark extract of Acanthopanax senticosus on the production of superoxide anion and hydrogen peroxide in mouse peritoneal macrophages in vitro and in vivo . Exposure of mouse peritoneal macrophages to A. senticosus extract significantly suppressed superoxide anion production induced by zymosan in a dose‐dependent manner. Similarly, exposure of mouse peritoneal macrophages to A. senticosus extract significantly inhibited hydrogen peroxide production induced by phorbol 12‐myristate 13‐acetate (PMA) in a dose‐dependent manner. Intraperitoneal administration of A. senticosus extract to KM mice reduced the ex vivo production of zymosan induced‐superoxide anion and PMA‐induced hydrogen peroxide by their peritoneal macrophages. Exposure to A. senticosus extract did not affect the cell viability or systemic toxicity. A. senticosus inhibited reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo and may be partly responsible for the antiinflammatory function. Copyright © 2008 John Wiley & Sons, Ltd.